Detection of Chlamydophila felis and Feline Herpesvirus by Multiplex Real-Time PCR Analysis
نویسندگان
چکیده
منابع مشابه
Detection of Chlamydophila felis and feline herpesvirus by multiplex real-time PCR analysis.
Chlamydophila felis and feline herpesvirus (FHV) are pathogens commonly associated with feline respiratory and ocular disease. A real-time multiplex PCR assay was developed to allow detection of these organisms, together with feline 28S ribosomal DNA, in a single tube. Of 538 ocular swab samples tested, 123 were positive for FHV, 97 were positive for C. felis, and 16 were positive for both path...
متن کاملoccurrence of chlamydophila felis, feline herpesvirus 1 and calcivirus in domestic cats of iran
background and objectives: feline herpesvirus-1, feline calicivirus and chlamydophila felis are the main causes of feline upper respiratory tract disease. this study was conducted to identify of fehv-1, fcv and c. felis infections in domestic cat population and also to estimate the prevalence of each specific infection in iran. materials and methods: the ocular conjunctiva and oropharyngeal spe...
متن کاملOccurrence of Chlamydophila felis, feline herpesvirus 1 and calcivirus in domestic cats of Iran
BACKGROUND AND OBJECTIVES Feline herpesvirus-1, feline calicivirus and Chlamydophila felis are the main causes of feline upper respiratory tract disease. This study was conducted to identify of FeHV-1, FCV and C. felis infections in domestic cat population and also to estimate the prevalence of each specific infection in Iran. MATERIALS AND METHODS The ocular conjunctiva and oropharyngeal spe...
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OBJECTIVES To develop a single multiplex real-time PCR assay to detect six different genetic types of carbapenemases already identified in Enterobacteriaceae (KPC, GES, NDM, IMP, VIM and OXA-48). METHODS A total of 58 bacterial isolates were tested. Thirty were previously characterized as resistant to carbapenems and documented by PCR and sequencing analysis to carry the following genes: bla(...
متن کاملDetection and discrimination of two Brucella species by multiplex real-time PCR and high-resolution melt analysis curve from human blood and comparison of results using RFLP
Objective(s): Rapid and accurate detection of Brucella abortus and Brucella melitensis from clinical samples is so important because antibiotic treatment has major side effects. This study reveals a new method in detection of clinical samples of brucellosis using real-time PCR and high-resolution melt (HRM) curve analysis. Materials and Methods: 160 brucellosis suspicious samples with more tha...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2003
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.41.6.2734-2736.2003